CpG-ODN enhances ingestion of apoptotic neutrophils by macrophages
Identifieur interne : 001A16 ( Main/Exploration ); précédent : 001A15; suivant : 001A17CpG-ODN enhances ingestion of apoptotic neutrophils by macrophages
Auteurs : Jiong Wang [République populaire de Chine] ; Wei-Lin Huang [République populaire de Chine] ; Rong-Yu Liu [République populaire de Chine]Source :
- Clinical and Experimental Medicine [ 1591-8890 ] ; 2009-03-01.
English descriptors
- KwdEn :
Abstract
Abstract: The clearance of apoptotic neutrophils by macrophages plays an important role in the process of inflammatory response. In the present study, we examined the ability of macrophages to ingest apoptotic neutrophils after activated by synthetic oligodeoxynucleotides containing CpG motifs (CpG-ODN) in vitro. The results showed that, while CpG-ODN at the experimental concentration had no cytotoxic effect on the viability of macrophages, the percentage of macrophages with ingested apoptotic neutrophils was increased from 23.6 to 42.30% by CpG-ODN stimulation. This effect was silenced when macrophages were treated with the mutation of CpG-ODN motifs. Both the total and cell surface protein of Toll-like receptor 9 (TLR9) expression in macrophages was up-regulated after CpG-ODN stimulation. While chloroquine (CHQ) had no effect on TLR9 expression in macrophages, it abolished the enhanced uptake of apoptotic neutrophils by macrophages. Although CpG-ODN had no significant effect on the IL-6 production, it was able to induce the increase of TNF-α protein expression and this effect was inhibited by CHQ pretreatment. Increased TNF-α production from macrophages induced by CpG-ODN stimulation was down-regulated after phagocytosis of apoptotic neutrophils. In conclusion, CpG-ODN could enhance the ingestion of apoptotic neutrophils by macrophages via TLR9 accompanied with an increasing in the level of TNF-α. After phagocytosis of apoptotic neutrophils, the increased TNF-α production from macrophages induced by CpG-ODN stimulation was down-regulated which the implications in the immune response remains for the further study.
Url:
DOI: 10.1007/s10238-008-0017-x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000518
- to stream Istex, to step Curation: 000518
- to stream Istex, to step Checkpoint: 000921
- to stream Main, to step Merge: 001A24
- to stream Main, to step Curation: 001A16
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">CpG-ODN enhances ingestion of apoptotic neutrophils by macrophages</title><author><name sortKey="Wang, Jiong" sort="Wang, Jiong" uniqKey="Wang J" first="Jiong" last="Wang">Jiong Wang</name></author><author><name sortKey="Huang, Wei Lin" sort="Huang, Wei Lin" uniqKey="Huang W" first="Wei-Lin" last="Huang">Wei-Lin Huang</name></author><author><name sortKey="Liu, Rong Yu" sort="Liu, Rong Yu" uniqKey="Liu R" first="Rong-Yu" last="Liu">Rong-Yu Liu</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E2132B94131990F7CE6A8603F7391FE716F3D2A5</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1007/s10238-008-0017-x</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-TQ596LG9-2/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000518</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000518</idno><idno type="wicri:Area/Istex/Curation">000518</idno><idno type="wicri:Area/Istex/Checkpoint">000921</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000921</idno><idno type="wicri:doubleKey">1591-8890:2008:Wang J:cpg:odn:enhances</idno><idno type="wicri:Area/Main/Merge">001A24</idno><idno type="wicri:Area/Main/Curation">001A16</idno><idno type="wicri:Area/Main/Exploration">001A16</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">CpG-ODN enhances ingestion of apoptotic neutrophils by macrophages</title><author><name sortKey="Wang, Jiong" sort="Wang, Jiong" uniqKey="Wang J" first="Jiong" last="Wang">Jiong Wang</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pulmonary, Anhui Geriatrics Institute, The First Affiliated Hospital of Anhui Medical University, Jixi Road 218, 230022, Hefei</wicri:regionArea><placeName><settlement type="city">Hefei</settlement><region type="province">Anhui</region></placeName></affiliation></author><author><name sortKey="Huang, Wei Lin" sort="Huang, Wei Lin" uniqKey="Huang W" first="Wei-Lin" last="Huang">Wei-Lin Huang</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pulmonary, Anhui Geriatrics Institute, The First Affiliated Hospital of Anhui Medical University, Jixi Road 218, 230022, Hefei</wicri:regionArea><placeName><settlement type="city">Hefei</settlement><region type="province">Anhui</region></placeName></affiliation></author><author><name sortKey="Liu, Rong Yu" sort="Liu, Rong Yu" uniqKey="Liu R" first="Rong-Yu" last="Liu">Rong-Yu Liu</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pulmonary, Anhui Geriatrics Institute, The First Affiliated Hospital of Anhui Medical University, Jixi Road 218, 230022, Hefei</wicri:regionArea><placeName><settlement type="city">Hefei</settlement><region type="province">Anhui</region></placeName></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Clinical and Experimental Medicine</title><title level="j" type="abbrev">Clin Exp Med</title><idno type="ISSN">1591-8890</idno><idno type="eISSN">1591-9528</idno><imprint><publisher>Springer Milan</publisher><pubPlace>Milan</pubPlace><date type="published" when="2009-03-01">2009-03-01</date><biblScope unit="volume">9</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="37">37</biblScope><biblScope unit="page" to="43">43</biblScope></imprint><idno type="ISSN">1591-8890</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1591-8890</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Apoptosis</term><term>CpG motifs</term><term>Engulfment</term><term>Macrophage</term><term>TLR9</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The clearance of apoptotic neutrophils by macrophages plays an important role in the process of inflammatory response. In the present study, we examined the ability of macrophages to ingest apoptotic neutrophils after activated by synthetic oligodeoxynucleotides containing CpG motifs (CpG-ODN) in vitro. The results showed that, while CpG-ODN at the experimental concentration had no cytotoxic effect on the viability of macrophages, the percentage of macrophages with ingested apoptotic neutrophils was increased from 23.6 to 42.30% by CpG-ODN stimulation. This effect was silenced when macrophages were treated with the mutation of CpG-ODN motifs. Both the total and cell surface protein of Toll-like receptor 9 (TLR9) expression in macrophages was up-regulated after CpG-ODN stimulation. While chloroquine (CHQ) had no effect on TLR9 expression in macrophages, it abolished the enhanced uptake of apoptotic neutrophils by macrophages. Although CpG-ODN had no significant effect on the IL-6 production, it was able to induce the increase of TNF-α protein expression and this effect was inhibited by CHQ pretreatment. Increased TNF-α production from macrophages induced by CpG-ODN stimulation was down-regulated after phagocytosis of apoptotic neutrophils. In conclusion, CpG-ODN could enhance the ingestion of apoptotic neutrophils by macrophages via TLR9 accompanied with an increasing in the level of TNF-α. After phagocytosis of apoptotic neutrophils, the increased TNF-α production from macrophages induced by CpG-ODN stimulation was down-regulated which the implications in the immune response remains for the further study.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Anhui</li></region><settlement><li>Hefei</li></settlement></list><tree><country name="République populaire de Chine"><region name="Anhui"><name sortKey="Wang, Jiong" sort="Wang, Jiong" uniqKey="Wang J" first="Jiong" last="Wang">Jiong Wang</name></region><name sortKey="Huang, Wei Lin" sort="Huang, Wei Lin" uniqKey="Huang W" first="Wei-Lin" last="Huang">Wei-Lin Huang</name><name sortKey="Liu, Rong Yu" sort="Liu, Rong Yu" uniqKey="Liu R" first="Rong-Yu" last="Liu">Rong-Yu Liu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A16 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001A16 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:E2132B94131990F7CE6A8603F7391FE716F3D2A5
|texte= CpG-ODN enhances ingestion of apoptotic neutrophils by macrophages
}}
| This area was generated with Dilib version V0.6.33. |  |